2D-DIGE to identify proteins associated with gestational diabetes in omental adipose tissue

K Oliva; G Barker; GE Rice; MJ Bailey; M Lappas

Journal article

2D-DIGE to identify proteins associated with gestational diabetes in omental adipose tissue

K Oliva, G Barker, GE Rice, MJ Bailey, M Lappas

Journal of Endocrinology | BIOSCIENTIFICA LTD | Published : 2013

DOI: 10.1530/JOE-13-0010

Abstract

Gestational diabetes mellitus (GDM) is a significant risk factor for the type 2 diabetes epidemic in many populations. Maternal adipose tissue plays a central role in the pathophysiology of GDM. Thus, the aim of this study was to determine the effect of GDM on the proteome of adipose tissue. Omental adipose tissue was obtained at the time of term Caesarean section from women with normal glucose tolerance (NGT) or GDM. 2D-difference gel electrophoresis (DIGE), followed by mass spectrometry, was used to identify protein spots (n=6 patients per group). Western blotting was used for confirmation of six of the spot differences (n=6 patients per group). We found 14 proteins that were differentiall..

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University of Melbourne Researchers

Martha Lappas Author

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Grants

Awarded by National Health and Medical Research Council (NHMRC)

Awarded by Australian NHMRC

Funding Acknowledgements

Dr M L was a recipient of Career Development Fellowships from National Health and Medical Research Council (NHMRC) (grant no. 454777 and 1047025). Prof. G E R was in receipt of an NHMRC Principal Research Fellowship. The work described in this manuscript was funded by project grants from NHMRC (grant no. 454310) and Diabetes Australia Research Trust (DART). Proteomic data analysis described in this work was supported by the use of the Australian Proteomics Computational Facility funded by the Australian NHMRC (grant no. 381413). Funding for the ChemiDoc system was, in part, provided by the Medical Research Foundation for Women and Babies.